Figure 5.

CircHECTD1 controlled cell proliferation and migration by binding miR-320-5p to regulate SLC2A1 expression. (A, B) RT-qPCR analysis and western blot of the relative abundance of SLC2A1 expression. (C, D) RT-qPCR and western blot analyses of the proliferation marker genes. (E) Representative micrographs and quantification of the EdU assay. Nuclei were stained with Hoechst dye. Bar = 200μm. (F) Flow cytometric measurement of the cell cycle distribution. (G) Representative micrographs and quantification of crystal violet-stained cell colonies. (H) The cellular migration was determined by the Transwell assay. Bar = 200μm. (I) After transfection, photographs were taken when the wound was created at 0 h, 24 h, 48 h, and 72 h in C6 cells, and the percentages of migrated cells were calculated. Bar = 400 μm. The data represent the mean ± SEM of three independent experiments. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001 compared with the control.