Figure 6.

CircHECTD1 enhanced gliobastoma multiform growth in vivo. (A) Representative images in immunodeficient mice. (B) The tumor weights of immunodeficient mice injected with C6 cells. (C) Representative micrographs and quantification of Ki67 positive cells after transfection with circHECTD1 or the normal control. The nuclei were stained with Hoechst dye. Bar = 200μm. (D, E) The expression of SLC2A1 were detected by RT-qPCR and Western blot analyses. (F) Representative images and quantification of immunohistochemical staining for SLC2A1. The values are the mean ± SEM for three biological replicates. ^* P < 0.05; ^** P < 0.01; ^*** P < 0.001.