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. 2021 May 17;12:651105. doi: 10.3389/fphys.2021.651105

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Copyright © 2021 Guo, Zhang, Wang, Huang, Liu, Chu, Ren, Kong and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Trx-1 on the gene and protein levels of Nrf2, the activation of NFκB and antioxidant defense parameters. (A) Representative protein expression bands of Nrf2. (B) Real-time quantitative PCR of Nrf2 mRNA levels in mice hippocampal tissue. (C) Representative Western blot analysis of Nrf2. (D) Antioxidant defense parameter: SOD activity analysis. (E) Antioxidant defense parameter: CAT activity analysis. (F) Antioxidant defense parameter: GSH-PX activity analysis. (G) Antioxidant defense parameter: MDA activity analysis. (H) Representative bands of the expression of NFκB. (I) Representative Western blot analysis of p-NFκB/NFκB. Tukey’s post hoc test for one-way ANOVA was used to analyze the mRNA levels, Western blot immunocontent, and antioxidant defense parameters. For each group, n = 6. The vertical lines represent the standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.