Table 1.

Comparison of NHS-muIL12 vs rmuIL-12 Effects on Markers of Immune Activation

Tissue	Assay	Cell	NHS-muIL12	rmuIL-12
Spleen	%BrdU	DC	++	+
		NK	++	±
		CD8	+	±
		Treg	±	±
		CD4/Foxp3-	±	±
	Class I	DC	++	NC
	Class II	DC	++	NC
Tumor	%BrdU	DC	NC	NC
		CD8	+	NC
		NK	++	NC
		Treg	NC	NC

Notes: C57BL/6 mice bearing MC38 s.c. tumors were injected with a single equimolar dose of either NHS-muIL12 or rmuIL-12. The listed immune cells were identified in the spleens/tumor by flow cytometry. Proliferation was measured by %BrdU incorporation and activation of dendritic cells by MHC expression levels. Results were quantified as follows: NC, no change; ±, < 10% increase; +, 10–25% increase; ++, >25% increase. Data from Xu et al¹⁰ and Fallon et al²².

Abbreviations: DC, dendritic cells; NK, natural killer; s.c., subcutaneous; Treg, regulatory T cells.