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Schematic representation of the Q-PCR technique used to evaluate the mtDNAT414G/mtDNA ratio. Primer set F5/R4 is designed to amplify total mtDNA. The primer set R4/MutF3 specifically detects mtDNA molecules carrying the T414G mutation. The primer contains the T414G mutation in order to amplify only the mtDNA containing a guanine in position 414.
