Figure 4.

mtDNA^T414G/total mtDNA ratio analysis in the different corneal cellular layers. The mtDNA^T414G/total mtDNA ratio was analysed by Q-PCR in the corneal epithelium, stroma and endothelium of 9 human subjects with a median age of 78 years old. We found that the mtDNA^T414G mutation is concentrated in the stroma (0.043%), when compared to the epithelium (0.003%) and the endothelium (0.013%). According to Wilcoxon test, the mtDNA^T414G/total mtDNA ratio is significantly different between the corneal stroma and the epithelium and between the stroma and the endothelium (*P value < 0.04).