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Primer design for real-time polymerase chain reaction.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| BMP-2 | CCAAGATGAACACAGCTGGTCACAGA | CCCACGTCACTGAAGTCCACG |
| FGF2 | GGAGAAGAGCGACCCTCACATCA | GCCAGGTAACGGTTAGCACACACT |
| 18S rRNA | GACTCAACACGGGAAACCTCAC | CCAGACAAATCGCTCCACCAAC |
| BSP | CGAACAAGGCATAAACGGCACCA | CTCCATTGTCTTCTCCGCTGCT |
| OCN | CAGTTCTGCTCCTCTCCAGGCA | CATCCATAGGGCTGGGAGGTCA |
BMP-2, bone morphogenetic protein-2; FGF2, fibroblast growth factor 2; BSP, bone sialoprotein; OCN, osteocalcin.
