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The rates of apoptosis were analyzed through TUNEL assay (Kaiji Biotech, Nanjing, China). Apoptotic cells' percentage depended on TUNEL assay. The magnification was ×200. ∗P < 0.05: the ghrelin group in comparison with the control group; &P < 0.05: the perifosine group in comparison with the ghrelin group; #P < 0.05: the NS398 group in comparison with the perifosine group.
