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. 2021 May 28;9(5):e002432. doi: 10.1136/jitc-2021-002432

Figure 2.

Figure 2

iPSC-derived DCs (iPSC-DCs) activate antigen-specific CD8+ T cells in vitro. (A, B) Pmel-1 T cells (CD90.1+ CD8+) were isolated from splenocytes by CD8α positive selection, labeled with CFSE, and co-cultured with iPSC-DCs in the presence of influenza nucleoprotein (NP) epitope NP366–374 or hgp10025–33 and IL-2 (60 IU/mL). Two days later, cells were harvested for flow cytometric analysis. (A) Representative histogram showing CFSE dilution in Pmel-1 T cells. (B) Representative flow cytometric plots showing CD62L, CD25, and PD-1 expression in Pmel-1 T cells. Numbers denote per cent dividing CD62L−, CD25+, or PD-1+ cells. (C) Activated Pmel-1 T cells from the experiment (A, B) were co-cultured with hgp10025–33 or NP366–374 in the presence of antigen-presenting cells (splenocytes from C57BL/6 mice) for another 2 days. Representative flow cytometric plots show intracellular production of IFNγ, TNFα, and IL-2 in Pmel-1 T cells activated by iPSC-DCs. Numbers denote per cent positive cells. Data shown are representative of two independent experiments. CFSE, carboxyfluorescein succinimidyl ester; IFNγ, interferon gamma; IL-2, interleukin 2; PD-1, programmed cell death protein 1; TNFα, tumor necrosis factor alpha.