Figure 7.

Combining in situ administration of iPSC-DC with RT attenuates growth of distant untreated tumors.(A) Experimental set-up. (B) Frequency of peripheral blood effector memory CD44⁺ CD62L⁻ CD8⁺ T cells (Tem) in different treatment groups as indicated (n=6–7). (C) Tumor growth curves (mean) (left) and tumor weight (right) of distant untreated tumors in bilateral AT-3 tumor-bearing mice in different treatment as indicated (n=6–7). Individual tumor volume curves are shown in online supplemental figure 8. (D, E). Frequency of CD8⁺ T cells among CD45⁺ cells (D) and CD8⁺/CD11b⁺ cell ratio (E) in untreated distant AT-3 tumors in different treatment groups as indicated (n=6–7). (F) Representative images of immunohistochemistry for CD8 in untreated distant AT-3 tumors. Scale bars, 100 µm. Data panels show mean numbers of CD8⁺ cells per each high-power field (HPF) within five different areas. Each dot represents biologically independent mice (B–E). NS not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Tukey’s multiple comparisons. Data shown are representative of two independent experiments. Mean±SEM. ANOVA, analysis of variance; iPSC-DC, induced pluripotent stem cell-derived dendritic cells; i.t., intratumorally; RT, radiotherapy; TILs, tumor-infiltrating lymphocytes.