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Ex vivo immunization with paired peptides. PBMCs from HLA-A*02:01 positive healthy subjects were immunized ex vivo with viral peptides. After 14 days, IFNγ EliSpot assay was performed restimulating the cells with the same viral peptide or with the paired tumor-associated antigen (TAA) peptide. Individual (A) and average (B) responsiveness to each viral peptide. (C) Average cross-reactivity versus viral and paired TAA peptides. SFU = IFNγ spot forming units.
