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. 2021 May 31;11:11378. doi: 10.1038/s41598-021-90800-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Early termination mechanisms in mollusc-specific Delta2. (a) Delta1 and Delta2 gene structures and blast (DNAMAN) results. The protein sequences indicated in the purple frame are TM domains whereas those indicated in the black frame are common termination positions for Delta2. The sequences in the red frame were analysed in detail in (b). (b) Delta2 termination mechanisms in different molluscs. Red solid circles indicate branches containing Delta2. Red hollow circles indicate no Delta2. Both amino acid and nucleotide sequences are displayed here, and the left column includes the termination sequences in lophotrochozoan species whereas the right column denotes sequences after correction. The colours of amino acids represent the functions of codons; amino acids of the same colour have similar functions. The same colour nucleotide represents the same base. The amino acids with a dashed line corresponding to bases in the red frames and triangles represent mutation codon sites in Delta2. “*” means termination codon. MEGA X was used for visualizing mutation sites.