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. 2021 May 31;7:128. doi: 10.1038/s41420-021-00513-0

Fig. 5. Autophagic activities of BmAtg4 and BmAtg7 are accompanied by a variation of acetylation.

Fig. 5

A–A′ Protein levels of BmSqstm1, BmAtg3, and BmAtg8 (A) and immunofluorescent staining of BmAtg3 and BmAtg8 (A’) in the fat body after BmAtg4 RNAi for 24 h. Scale bar: 10 µm. B–B′ Protein levels of BmSqstm1, BmAtg3, and BmAtg8 (B) and immunofluorescent staining of BmAtg3 and BmAtg8 (B′) in the fat body after BmAtg7 RNAi for 24 h. Scale bar: 10 µm. C Acetylation levels of BmAtg4-V5 after starvation for 4 h. D Acetylation levels of BmAtg7-HA after starvation for 4 h. E–E′ Protein levels of BmAtg8, BmSqstm1, and BmAtg4-V5 (E), and immunofluorescent staining of BmAtg4-V5 (E′) after 20E or starvation treatment for 4 h, S starvation. Scale bar: 10 µm. F–F′ Protein levels of BmSqstm1, BmAtg7-HA, and BmAtg8 (F), and immunofluorescent staining of BmAtg7-HA (F′) after 20E or starvation treatment for 4 h. Scale bar: 10 µm. G Acetylation levels of BmAtg4-V5 after 5 μM 20E, starvation, 800 nM C646, 20 µM TSA, or 20 µM CTB treatment for 6 h in BmN cells. H Acetylation levels of BmAtg7-HA after 5 μM 20E, starvation, 800 nM C646, 20 µM TSA, or 20 µM CTB treatment for 6 h in BmN cells.