Skip to main content
. 2021 May 31;7:128. doi: 10.1038/s41420-021-00513-0

Fig. 6. Authenticity and autophagic activities of identified acetylation sites in BmAtg3, BmAtg4, and BmAtg8.

Fig. 6

A Acetylation level of BmAtg3 after single and double mutation of acetylation sites. 2KR: double acetylation-site mutated from lysine (K) to arginine (R). B Immunofluorescent staining of BmAtg3 double acetylation-site mutant. Scale bar: 10 µm. C–C′ Protein levels of BmSqstm1, BmAtg8, and BmAtg3 after overexpression of double acetylation-site mutated BmAtg3 under nutrient-rich conditions (C). Quantification of BmAtg8–PE in C (C′). Significance test was performed between egfp and mutant overexpression. D Acetylation level of BmAtg8 after single and sextuple mutation of acetylation sites. E Immunofluorescent staining of BmAtg8 after sextuple mutation of acetylation sites. 6KR: sextuple acetylation site mutation from lysine to arginine. Scale bar: 10 µm. F–F′ Protein levels of BmSqstm1, FLAG-BmAtg8, and endogenous BmAtg8 after overexpression of sextuple acetylation-site mutated BmAtg8 under nutrient-rich conditions (F). Quantification of BmAtg8–PE and EGFP- BmAtg8–PE in F (F′). G–G′ Acetylation of BmAtg4 after single and triple (G), or double (G′) mutation of acetylation sites. H Immunofluorescent staining of BmAtg4 after double mutation of acetylation sites. Scale bar: 10 µm. I–I′ Protein levels of BmSqstm1 and EGFP-BmAtg8 after overexpression of double acetylation-site mutated BmAtg4 under nutrient-rich conditions (I). Quantification of EGFP-BmAtg8–PE in I (I′). J Acetylation of c-Myc-BmAtg4, and protein levels of BmSqstm1 and BmHDAC1 after BmHDAC1 overexpression.