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. 2021 May 31;7:69. doi: 10.1038/s41523-021-00259-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Proliferation (a) and cell cycle (b) of LINC01119-overexpressing SUM159 cells compared to controls at the indicated days (mean ± SD of n = 3). c Left: representative images of colony-formation assays on the indicated MDA-MB-231 and SUM159 groups. Right: ImageJ quantitation of colony numbers in Left displayed as mean ± SD of n = 3. d Left: representative images of 3D anchorage-independent growth of the indicated MDA-MB-231 and SUM159 cell lines. Right: ImageJ quantitation of colony numbers in Left displayed as mean ± SD of n > 3. e Left: representative images of colony-formation assays on the indicated mouse 4T1 groups. Right: ImageJ quantitation of colony numbers in Left displayed as mean ± SD of n = 3. f Left: representative images of 3D anchorage-independent growth of the indicated mouse 67NR and 4T07 cell lines. Right: ImageJ quantitation of colony numbers in Left displayed as mean ± SD of n > 3. g Weight of the indicated SUM159 tumors grown orthotopically in NCG mice after 42 days (n > 5 per group). h Left: representative images of immunohistochemistry of Ki67 in tumor tissues in g. Right: quantitation of Ki67 positive cells in Left displayed as box-and-whisker plots representing the median (centerline) and inter-quartile range (IQR; box). The whiskers extend up to 1.5 times the IQR from the box to the smallest and largest points. i, j Proliferation (mean ± SD of n = 3) of Hs578T (i) and MDA-MB-468 (j) cells transfected with controls (NC) or with LINC01119 ASOs measured using WST-1 assay after 48 h. k Proliferation (mean ± SD of n = 3) of CAL51 cells transfected with control or with a combination of LINC01119 ASOs measured using CellTiter after 48 h.