Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 31;7:69. doi: 10.1038/s41523-021-00259-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Upper: schema of LINC01119 plasmid template for in-cell transcription/translation. Bottom: qRTPCR (mean ± SD of n = 3) (left) and Western blot (right) of RNA and lysates derived from HEK293T cells transiently transfected with LINC01119 or indicated controls. b Upper: schema of 5’ and 3’ LINC01119 primers utilized in in vitro transcription reactions. Bottom Left: in vitro transcribed LINC01119. Bottom Right: SDS-PAGE on in vitro translated luciferase, pTRI-Xef-1, and LINC01119. c Upper: schema of LINC01119 putative coding sequences. Bottom: Western blot analysis of lysates of HEK293T cells transiently transfected with empty pFLAG-CMV-1, pFLAG-CMV-1-Vimentin, PCS#1 and #4. d Upper: schema of 5’ and 3’ LINC01119 PCS#1 with primers utilized in in vitro transcription reactions. Bottom Left: Luciferase activity (mean ± SD of n = 3) of the successfully transcribed and translated luciferase used as a positive control for transcription. Bottom Right: SDS-PAGE on in vitro translated luciferase, pTRI-Xef-1, and LINC01119 PCS#1. e, f Localization of LINC01119 in control MDA-MB-231, MDA-MB-468, and SUM149 cells (e) and in HCC1937, MDA-MB-231 and SUM159 cells stably over-expressing LINC01119 (f) determined by RNAscope; dapB and Hs-PPIB served as negative and positive controls, respectively. Images shown are representatives of n > 3.