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. 2020 Nov 30;28(5):1477–1492. doi: 10.1038/s41418-020-00672-0

Fig. 4. TP53 variants found in the human population do not display loss of activity.

Fig. 4

Transcriptional activity analysis: WAF1 (A), MDM2 (B), and CONS (C) promoters upstream of the luciferase reporter were transiently transfected in H1299 cells with a range of TP53 variants. Luciferase activity in the cell lysates was determined at 24 h after transfection (see also Fig. S6 for other TP53 variants). Colony growth arrest: H1299 cells were transfected with various TP53 variants and selected for 2 weeks in a selective medium with G418 before staining with crystal violet. Mock: non-transfected cells. TP53 variants with impaired activities (p.R175H and p.I254T) were used as controls. Two independent experiments are shown in this figure (D, E). Variants from set 21 are shown in green. Other experiments and quantitative analyses of colony numbers are available in Fig. S8.