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. 2021 May 31;12(6):563. doi: 10.1038/s41419-021-03843-0

Fig. 5. Epithelial PBLD deficiency recruits more immune cells infiltration in murine colitis.

Fig. 5

a Myeloperoxidase (MPO) and (b) F4/80-stained colonic tissue sections in mice. Images are representative from three to four mice in each group. Scale bars: 200 μm in upper in both (a and b), 50 μm in lower in both (a and b). c Cells isolated from colonic lamina propria or mesenteric lymph nodes were labeled with anti-F4/80 and anti-CD11b, d anti-Ly-6C and anti-Ly-6G, and then visualized via flow cytometry and e their corresponding proportions of macrophages, monocytes, and neutrophils in CLP or MLN. WT, n = 3; DSS:WT, n = 5; DSS:PBLDIEC−/−, n = 5. CLP colonic lamina propria, MLN mesenteric lymph node. f Enzyme-linked immunosorbent assay (ELISA) of whole-colon homogenates in mice to evaluate TNF-α, IL-6, IFN-γ, and IL-1β production (n = 4). Data are mean ± standard deviation (SD). *p < 0.05.