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. 2021 May 31;11:11372. doi: 10.1038/s41598-021-90779-2

Figure 5.

Figure 5

AMPK mediated CTRP9-induced NO production and reduced ET-1 production by activating eNOS and inactivating ERK1/2. (A–C) Western blotting of expression of AMPK and p-AMPK (A, n = 3; blots were cropped from different parts of the same gel), eNOS and p-eNOS (B, n = 3; blots are cropped from different gels), ERK1/2 and p-ERK1/2 (C, n = 3; blots cropped from different gels) in rat PMVECs. (D, E) Levels of NO (nN+AAV-Control = 3, nN+AAV-CTRP9 = 3, nH+AAV-Control = 5, nH+AAV-CTRP9 = 4) and ET-1 (nN+AAV-Control = 5, nN+AAV-CTRP9 = 5, nH+AAV-Control = 5, nH+AAV-CTRP9 = 6) in PMVECs culture medium. Data are the mean ± SEM. ※P < 0.05 vs. group N; #P < 0.05 vs. group H; &P < 0.05 vs. group H + CTRP9. H hypoxia, N normoxia, C.C compound C.