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. 2021 May 31;11:11386. doi: 10.1038/s41598-021-90941-w

Figure 2.

Figure 2

Glypican-1 knockout mice have inhibited eNOS phosphorylation in both chronic and increased flow conditions. Descending aorta from WT and Gpc1−/− mice were en face immunostained for peNOS and PECAM-1. (A) Mean normalized peNOS expression under chronic flow is shown for WT (n = 10) and Gpc1−/− mice (n = 10). *P < 0.05 using two-tailed student’s t-test. (B) Mean normalized PECAM-1 expression under chronic flow is shown for WT (n = 6) and Gpc1−/− (n = 5) mice. *P < 0.05 using two-tailed student’s t-test. (C) Mean normalized peNOS expression under increased flow induced by dobutamine for 30 min is shown for WT (n = 9 and 8 for saline and dobutamine groups, respectively) and Gpc1−/− mice (n = 8 for both saline and dobutamine groups). *P < 0.05 using one-way ANOVA followed by Sidak’s multiple comparisons test.