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. 2021 May 31;11:11386. doi: 10.1038/s41598-021-90941-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PECAM-1 knockdown blocks shear-induced NO production at 30 min in HUVEC. (A) PECAM1 gene expression is shown in cells transfected with negative control siRNA and with siRNA specific to PECAM1 mRNA (n = 10 for each group). Gene expression was normalized to GAPDH and β-ACTIN expression levels and both transfection groups were normalized to the negative control expression data, set to 100% expression. *P < 0.05 using two-tailed student’s t-test. (B) GPC1 gene expression is shown in transfected cells (n = 4 for each group). Expression levels did not significantly change using two-tailed student’s t-test. (C) PECAM-1 protein expression in transfected cells (n = 3 for each group) and corresponding western blot. *P < 0.05 using two-tailed student’s t-test. Full length blot is presented in Supplemental Figure S8. (D) DAF-2T normalized levels are shown for transfected HUVEC exposed to 30 min of fluid shear stress. From left to right, n = 8, 16, 8, 16. *P < 0.05 using one-way ANOVA followed by Sidak’s multiple comparisons test.