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Heparin and Surfen treatment does not affect shear-induced NO production at 30 min in HUVEC. Heparin (500 µg/mL) and surfen (10 µM) were used to treat HUVEC 15 min prior to fluid shear stress exposure to block shear-induced HS binding to PECAM-1. DAF-2T levels were normalized to static control levels within each set. From left to right, n = 17, 22, 8, 15, 12, 16. *P < 0.05 using one-way ANOVA followed by Sidak’s multiple comparisons test.
