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. 2021 May 31;11:11386. doi: 10.1038/s41598-021-90941-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HS cleavage blocks shear-induced PECAM-1 tyrosine kinase phosphorylation at 30 min in HUVEC. Heparinase III (1215 mU/mL) was used to cleave HS from HUVEC monolayers 2 h prior to fluid shear stress exposure. (A) PECAM-1 phosphorylation levels were measured using western blotting for total tyrosine phosphorylation (following PECAM-1 immunoprecipitation) and (B) for PECAM-1 specific tyrosine 686 phosphorylation. Full length blots are presented in Supplemental Figure S8. Densitometry values from both phosphorylation types were normalized to PECAM-1 expression in each sample and then normalized to untreated static control levels within each set. From left to right within each set, (A) n = 6, 13, 5, 14 for total tyrosine phosphorylation (B) n = 6, 12, 6, 12 for tyrosine 686 phosphorylation. Results in each graph are averaged from 5 or more independent experiments. *P < 0.05 using one-way ANOVA followed by Sidak’s multiple comparisons test.