Fig. 3.

DNA-NETs promote arteritis progression. (A) Fluorescence microscope for NETs (green) of PAECs cocultured with or without PMA-stimulated and with or without DNase Ⅰ-treated neutrophils (scale bar, 50 μm). Fields from one representative experiment of three are shown. (B) Quantification of NET formation by fluorescence microscope in PAECs cocultured with or without PMA-stimulated and with or without DNase Ⅰ-treated neutrophils (N = 3; Groups that do not share the same letters are significantly different (P < 0.05) from each other). (C) IF analyses of TNF-α (red) and IL-1β (green) in PAECs cocultured with or without PMA-stimulated and with or without DNase Ⅰ-treated neutrophils (scale bar, 20 μm). Fields from one representative experiment of three are shown. (D) Quantification of relative TNF-α and IL-1β intensity in PAECs cocultured with or without PMA-stimulated and with or without DNase Ⅰ-treated neutrophils (N = 3; Bars that do not share the same letters are significantly different (P < 0.05) from each other). Results are presented as mean ± SEM. Statistical significance was obtained by one-way ANOVA or unpaired Student's t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)