Table 1.
Differential phenotypic characteristics of strains AF52-21T, CM04-06T, and the related species F. prausnitzii ATCC 27768T.
| Phenotypic features | 1 | 2 | 3 |
|---|---|---|---|
| Growth | |||
| Temperature range (optimum) (°C) | 20–42 (37) | 30–45 (37) | 20–42 (37) |
| pH range | 6.0–7.5 | 5.0–8.0 | 6.0–7.5 |
| Salt tolerance (%) | 1 | 3 | 3 |
| Fermentation products | |||
| formic acid (mM) | 4.86 | 7.62 | 18.20 |
| acetic acid (mM) | 69.70 | 44.8 | 29.67 |
| butyric acid (mM) | 15.08 | 40.03 | 39.10 |
| lactic acid (mM) | 29.25 | 30.53 | 5.70 |
| Hydrolysis of | |||
| Aesculin | + | − | + |
| Gelatin | − | + | − |
| Acid from (API 20A and API 50CHL) | |||
| Cellobiose | + | − | w |
| D-Fructose | w | − | + |
| D-Fucose | w | − | w |
| D-Galactose | w | − | − |
| D-Glucose | w | − | + |
| D-Lactose | + | − | − |
| D-Maltose | + | + | w |
| D-Mannitol | + | − | − |
| D-Mannose | + | + | − |
| D-Raffinose | − | w | − |
| D-Trehalose | + | w | w |
| Gluconate | − | − | + |
| Glycogen | + | − | − |
| Inositol | w | − | − |
| Inulin | + | − | + |
| Methyl-β-D-Xylopyranoside | w | − | − |
| Enzyme activity (API ZYM) | |||
| N-acetyl-β-Glucosaminidase | − | w | − |
| Naphthol-AS-BI-Phosphohydrolase | + | − | + |
| α-Glucosidase | − | − | + |
| β-Galactosidase | − | − | w |
| β-Glucosidase | + | − | − |
| β-Glucuronidase | + | w | + |
| DNA G + C (mol %) | 57.77 | 57.51 | 52 − 57 |
Strains: 1, F. butyricigenerans AF52-21T; 2, F. longum CM04-06T; 3, F. prausnitzii ATCC 27768T. + , positive; w, weakly positive; −, negative. All data are from this study.