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. 2021 May 12;12:e00174. doi: 10.1016/j.mec.2021.e00174

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Comparison of CcpA, HPr and PtsG between ΔsrfA and WH1. A and B: Difference of CcpA, HPr and PtsG between ΔsrfA and WH1 at the level of transcription (A) and protein (C), respectively. Red: up-regulation; Green: down-regulation. B: Verification of the transcription of ccpA, hpr, hprK and hprP by qRT-PCR. D: Verification of CcpA and HPr by Western blot. The purified recombinant CcpA and HPr was used as control. Due to introduction of an extra sequence from the pET28a vector, the recombinant CcpA and HPr showed a higher molecular weight than the proteins in WH1 and ΔsrfA. E: Verification of P ~ Ser₄₆-HPr by Western blot. Red arrows direct the objective proteins. Single and double stars represent significant (p < 0.05) and very significant (p < 0.01) difference between two groups, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 — Comparison of CcpA, HPr and PtsG between ΔsrfA and WH1. A and B: Difference of CcpA, HPr and PtsG between ΔsrfA and WH1 at the level of transcription (A) and protein (C), respectively. Red: up-regulation; Green: down-regulation. B: Verification of the transcription of ccpA, hpr, hprK and hprP by qRT-PCR. D: Verification of CcpA and HPr by Western blot. The purified recombinant CcpA and HPr was used as control. Due to introduction of an extra sequence from the pET28a vector, the recombinant CcpA and HPr showed a higher molecular weight than the proteins in WH1 and ΔsrfA. E: Verification of P ~ Ser₄₆-HPr by Western blot. Red arrows direct the objective proteins. Single and double stars represent significant (p < 0.05) and very significant (p < 0.01) difference between two groups, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)