Fig. 3.

B7-H7 inhibits T cells in the presence of TCR and CD28 signaling. a Naive CD4 T cells were purified and stimulated with OKT3 + B7-1 Fc, OKT3 + B7-1 Fc + Ctrl Fc, and OKT3 + B7-1 Fc + B7-H7 Fc for 2 h. RNA was isolated and whole-transcriptome sequencing was performed. Heatmap of genes that are significantly regulated in RNA-sequencing studies. OKT3 + B7-1 Fc-treated cells were used as the reference sample in the generation of the heatmap (n = 3 donors, fold change >4, FDR <0.25). b CD25 and PD-1 protein expression on naive CD4 T cells after 72 h stimulation under indicated conditions. c Cell culture supernatants were collected at 72 h, and IL-2 and IFNγ were measured. d CFSE dilution was measured in the presence of plate-bound OKT3 + B7-1 Fc, OKT3 + B7-1 Fc + Ctrl Fc, and OKT3 + B7-1 Fc + B7-H7 Fc. Bar plot summarizes data from three independent experiments. e Naive CD4 T cells were stimulated with beads coated with indicated reagents and proliferation was measured 72 h later by flow cytometry. (***p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.005, *p ≤ 0.05)