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. 2021 May 31;11:11359. doi: 10.1038/s41598-021-90930-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The 3D Surface Plot (Negative Exponential Smoothing), a 3D distribution surface was constructed, which characterizes the dependence of the number of detected RLNs with metastases on concentrations of various pairs of cytokines (pg/ml) produced by IC-NST samples. (a) The dependence of the number of detected RLNs with metastases on concentrations of TNF-α and GM-CSF produced by IC-NST samples. (b) The dependence of the number of detected RLNs with metastases on concentrations of IL-10 and IL-17 produced by IC-NST samples. (c) The dependence of the number of detected RLNs with metastases on concentrations of IL-18 and VEGF-A produced by IC-NST samples. (d) The dependence of the number of detected RLNs with metastases on concentrations of IL-18 and IFN-γ produced by IC-NST samples.