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. 2021 May 31;11:11376. doi: 10.1038/s41598-021-89765-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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High glucose promoted mesenchymal phenotype and induced motility and invasion in HCC cell lines. Immunofluorescence imaging of Alexa-488-conjugated Phalloidin labeling of F-actin stress fibrils in (a) HuH-7 and (b) SNU-449 cells in no- and high-glucose (25 mM) supplemented culture conditions. Immunoblotting of cell cytoskeleton EMT biomarkers N-cadherin and Vimentin expressions in (c) HuH-7, (d) SNU-449 and (e) SK-HEP-1 cells. Graphical presentation of fold difference in motility and invasion of (f) HuH-7, (g) SNU-449 and (h) SK-HEP-1 cells cultured in no- and high-glucose supplemented media analyzed by trans-well motility and invasion assays. Graphical presentation of (i, left) wound closure percentage of SNU-449 cells after 6-, 12-, 18- and 24-h incubation in no- and high-glucose supplemented culture conditions. Wound-healing graphical presentation represents 3 independent experiments. Combined microscopic images of (i, right) SNU-449 wound healing assay from automated microscope for real-time live cell imaging on 0, 6th, 12th and 24th hours. Full length blots are presented in Supplementary. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. Statistical analyses were performed and column graphs were generated using GraphPad Prism version 8.2.1 for MacOS, GraphPad Software, San Diego, California USA, https://www.graphpad.com .* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.