Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 31;11:11376. doi: 10.1038/s41598-021-89765-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

High glucose did not induce glucose toxicity but enhanced spheroid formation in HCC cells. Analysis of toxicity of (a) HuH-7, (b) SNU-449, (c) HepG2 and (d) SK-HEP-1 cells in response to increasing glucose concentrations (0, 5, 25, 50 mM) with MTT assay. Cell survival is represented as absorbance (570 nm). Analyses of cell survival differences of (e) HuH-7, (f) SNU-449, (g) HepG2 and (h) SK-HEP-1 cells in no-, normo- (5.5 mM) and hyperglycemic-glucose (25 mM) conditions by SRB. Brightfield images and graphical presentation of hanging-drop spheroid formation assay performed with (i) HuH-7 and (j) SNU-449 cells in no- and high-glucose (25 mM glucose) supplemented conditions. Spheroid area is calculated with ImageJ. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. Statistical analyses were performed and graphs were generated using GraphPad Prism version 8.2.1 for MacOS, GraphPad Software, San Diego, California USA, https://www.graphpad.com. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.