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. 2021 May 31;11:11376. doi: 10.1038/s41598-021-89765-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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High glucose increased cell aggressiveness reversed by inhibition of c-Met activation. Representative images showing protein expression of c-Met (Alexa-594 labeled) and phospho-Met (Y1234/Y1235), (Alexa-488 labelled) in (a) HuH-7 and (b) SNU-449 cells. Representative images showing F-actin stress fibrils (Alexa-488-conjugated Phalloidin labelled) in (c) HuH-7 and (d) SNU-449 cells. Cell nucleus is counterstained with DAPI staining. Graphical presentation of fold change of (e) motility and invasion in SNU-449 and (f) SK-HEP-1 cells in trans-well motility and invasion assays. Graphical presentation of (g) wound closure percentage of SNU-449 cells after 6-, 12-, 18- and 24-h incubation. Wound-healing graphical presentation represents 3 independent experiments. Combined microscopic images of SNU-449 wound healing assay from automated microscope for real-time live cell imaging on 0, 6th, 12th and 24th hours. Percentage of Annexin-V/PI positive HCC cells (h) in high glucose and c-Met inhibition conditions measured by flow cytometry. All data represented in this figure are from indicated HCC cells that are cultured with high-glucose control and high-glucose + SU11274 (2.5 µM) supplemented conditions. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. Statistical analyses were performed and graphs were generated using GraphPad Prism version 8.2.1 for MacOS, GraphPad Software, San Diego, California USA, https://www.graphpad.com. *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.