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. 2021 Apr 28;73(3):473–482. doi: 10.1007/s10616-021-00471-6

Fig. 6.

Fig. 6

Knockdown of NNT-AS1 expression inhibits glioma progression via miR-582-5p/EZH2 axis. a The RT-qPCR assay was implemented to certify transfection efficiency for miR-582-5p inhibitor and sh-EZH2. bd CCK-8, EdU, transwell assays were employed to estimate the role of NNT-AS1/miR-582-5p/EZH2 axis in U87MG cell proliferation, migration and invasion (magnification × 200). e, f RT-qPCR and western blot analyses were performed to detected mRNA and protein expressions of E-cadherin and N-cadherin. **P < 0.01 vs. inhibitor-NC, #P < 0.05, ##P < 0.01 vs. smiR-582-5p inhibitor