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. 2021 May 18;12:625699. doi: 10.3389/fphar.2021.625699

FIGURE 6.

FIGURE 6

Histological examination of the spinal cord of mice after chronic sulfasalazine (SAS) treatment. Representative photomicrographs of the eriochrome cyanine/neutral red staining (A) as well as the NeuN immunohistochemistry (B). A higher magnification picture of the boxed area in (B) is shown in (C). NeuN+ cells were quantified using the Cell Sens software (D). One slice of n = 3–5 mice/group was used, data are presented as mean ± SEM and analyzed using a Kruskal-Wallis test followed by Dunn’s multiple comparisons for each dose of SAS compared to saline.