Figure 4.

DMF treatment does not worsen the morphological alterations induced by EAE at the level of the ileum. Ilea were isolated from naïve mice and at 4 d post onset from EAE-affected mice treated or not with DMF. Top panels show representative XPCT images (longitudinal and transversal sections) of ileum in naïve, EAE-affected, and EAE-affected DMF-treated mice. Bottom panels show the quantification of the morphological parameters (length and transversal area) performed using ImageJ software (as described in Methods) for at least 120 villi/mouse (N = 3 mice per condition). Average clinical scores of mice at time of the analysis: WT-EAE-affected mice: 3 ± 0.17; WT-EAE-affected mice treated with DMF: 2.75 ± 0.11. Scale bars: 50 μm. Results are shown as mean ± SEM. ***P < 0.001.