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. 2021 May 18;12:652488. doi: 10.3389/fimmu.2021.652488

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Copyright © 2021 Janicova, Haag, Xu, Garza, Dunay, Neunaber, Nowak, Cavalli, Marzi, Sturm and Relja

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study design. Healthy volunteers (HV) received a standardized lunch one hour before starting the experiment and had one liter water at hand during the first observation period of 6 hours (T0-T6). Every 20 minutes, HV received an individually calculated amount of whisky-coke cocktail (EtOH) for four hours, reaching blood alcohol concentration of 1‰ after 4 hours (T4). Blood samples were collected before (T0) and 2 (T2), 4 (T4), 6 (T6), 24 (T24) and 48 (T48) hours after starting the alcohol consumption. This is the same study design as published by Sturm et al. (19).