Fig. 1. CD40 stimulation strongly activates NF-κB signaling and Bcl-XL expression in CLL.
CLL cells were cultured for 24 h on fibroblasts (3T3), fibroblasts transfected with human CD40L (3T40L), stimulated with CpG, or stimulated with IL-4 and αIgM. A Protein lysates were probed for p100, p52, p-p65, Bcl-XL, Bfl-1, and actin as loading control. B Densitometric analysis of p100, p52, p-p65, Bcl-XL, and Bfl-1 are shown normalized to the actin loading control (n = 7). Bars represent the mean ± SEM, *p < 0.05, **p < 0.01 (two-way ANOVA).