Fig. 5.

OTUB1 deletion in DCs leads to impaired parasite control in toxoplasmosis. a–c OTUB1^fl/fl and CD11c-Cre OTUB1^fl/fl mice were infected i.p. with 50,000 tachyzoites. Peritoneal cells were isolated from the peritoneal cavities and analyzed by flow cytometry at 2 and 4 days p.i. a Percentages of infected CD11c⁺ CD8a⁺, CD11c⁺ PDCA-1⁺, and CD11c⁺ CD11b⁺ DCs. b Percentages of infected peritoneal CD45⁺ cells in OTUB1^fl/fl and CD11c-Cre OTUB1^fl/fl mice. c Percentages of infected peritoneal leukocyte subpopulations in OTUB1^fl/fl and CD11c-Cre OTUB1^fl/fl mice. Representative FACS plots (upper panel) and statistics (lower panel) are shown (n = 10 for each group). d Mice were infected i.p. with 50,000 tachyzoites for 10 days. Parasite loads in different organs were determined by semiquantitative PCR of tissue DNA (n = 5 for each group). e, f Mice were infected i.p. with three cysts. Parasite load in the brain 30 days p.i. was determined by semiquantitative PCR of tissue DNA (n = 4 for each group) (e). Survival was monitored for 70 days p.i. (f) (n = 10 for each group). Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001; n.s. not significant