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. 2020 Dec 7;28(5):1705–1719. doi: 10.1038/s41418-020-00695-7

Fig. 3. Hedgehog/Gli1 signaling is required for the regulation of NEK7/NLRP3 and RIPK3 activation in myeloid Foxo1-deficient livers in response to IR stress.

Fig. 3

A Immunofluorescence staining of AlexaFluor488-labeled control siRNA and CD68-positive macrophages in ischemic liver tissue (n = 3–4 mice/group), Scale bars, 40 and 10 μm. B Representative histological staining (H&E) of ischemic liver tissue (n = 4–6 mice/group) and Suzuki’s histological score. Scale bars, 100 μm. C Serum ALT levels (IU/L; n = 4–6 samples/group). D Immunofluorescence staining of CD11b+ macrophages in ischemic livers (n = 4–6 mice/group). Quantification of CD11b+ macrophages, Scale bars, 40 μm. E Immunohistochemistry staining of Ly6G+ neutrophils in ischemic livers (n = 4–6 mice/group). Quantification of Ly6G+ neutrophils, Scale bars, 40 μm. F Western-assisted analysis and relative density ratio of Snail, NEK7, NLRP3, cleaved caspase-1, and RIPK3. Representative of three experiments. G ELISA analysis of serum IL-1β levels (n = 3–4 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.