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. 2020 Dec 7;28(5):1705–1719. doi: 10.1038/s41418-020-00695-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — BMMs were isolated from Foxo1^M-KO and Foxo1^FL/FL mice and transfected with the p-CRISPR-RIPK3 activation, p-CRISPR-RIPK3 KO, or control vector followed by LPS stimulation. A, D Western blots analysis and relative density ratio of RIPK3, NEK7, NLRP3, and cleaved caspase-1. B, E Immunofluorescence staining for the NLRP3 expression in macrophages. C, F ELISA analysis of supernatant IL-1β levels. All western blots represent three experiments, and 3–4 samples each group for immunofluorescence staining and ELISA assay. DAPI was used to visualize nuclei. Scale bars, 40 and 20 μm. The data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.