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. 2020 Dec 7;28(5):1705–1719. doi: 10.1038/s41418-020-00695-7

Fig. 8. Myeloid Foxo1 signaling-mediated Snail regulates RIPK3-mediated hepatocyte necroptosis during inflammatory response.

Fig. 8

BMMs were isolated from Foxo1M-KO mice and transfected with the p-CRISPR-Snail KO or control vector followed by LPS stimulation. A Western-assisted analysis and relative density ratio of HMGB1 in LPS-stimulated BMMs. Representative of three experiments. B ELISA analysis of supernatant HMGB1 levels in LPS-stimulated BMMs (n = 3–4 samples/group). C Schematic figure for macrophage/hepatocyte co-culture system. D BMMs transfected with the p-CRISPR-Snail KO were stimulated with LPS, and then co-cultured with primary hepatocytes that were supplemented with or without H2O2 for 24 h. LDH release from hepatocytes in co-cultures (n = 3–4 samples/group). E Western-assisted analysis and relative density ratio of RIPK3 and p-MLKL in hepatocytes after co-culture. Representative of three experiments. F Immunofluorescence staining of p-MLKL+ hepatocytes after co-culture (n = 4–6 mice/group). Scale bars, 40 μm. All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.