Fig. 2. Map-based cloning of the PvSL1 locus.

Using 124 INDEL markers, the PvSL1 locus was mapped to a 1675.3 kb region between PvM36 and PvM37 on chromosome 2. a Physical locations of fine-mapping markers and the number of recombinants defining the PvSL1 region. The PvSL1 locus was fine-mapped to the region between PvM359 and PvM357, in which six genes (Phvul.002G190500 to Phvul.002G191000) were annotated. b, c In the pvsl1 mutant, a single base was deleted at 1370 bp (A1370) in the third exon of Phvul.002G190900, leading to a frameshift mutation. In the gene model, exons, introns and UTRs are represented by black boxes, solid lines, and gray boxes, respectively. d The mutated Phvul.002G190900 protein retained the FtsH extracellular domain (red box) and ATPase domain (blue box) but lacked the Zn²⁺ metalloprotease domain (purple box) (Protease). e Expression levels of PvSL1 in Dalong1 and mutants under 100 and 75 μmol·m⁻²·s⁻¹ light conditions. f Expression levels of PvSL1 under 11, 75, and 250 μmol·m⁻²·s⁻¹ light conditions. e, f Unifoliate of 10-day-old seedlings were used to extract total RNA. Phvul.007G041700.1, the homologue of TIP41 in common bean, was used as the reference gene. Error bars indicated SD. g Subcellular localization of the PvSL1 and PvSL1 (ZMP⁻) proteins. Bars were 10 μm