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. 2021 May 31;12:3256. doi: 10.1038/s41467-021-23482-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative Western blots (top) and quantification (bottom) of iNOS, ARG1, and GAPDH of Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with media (Unstim.), IFNγ/LPS (0.3 ng/mL of each), or IL4/IL13 (0.1 ng/mL of each). b TNFα, IL6, and MCP1 secretion from Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with the indicated conditions, as measured by ELISA. c Relative gene expression of inflammatory and healing markers in Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with the indicated conditions, as measured by qPCR. Gene expression is shown relative to the highest expressing condition. d, e Representative Western blots (top) and quantification (bottom) of p-NFκB/NFκB of Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with media (Unstim.) or IFNγ/LPS for 1 h (d) and p-STAT6/STAT6 of Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with media (Unstim.) or IL4/IL13 for a period of 1 h (e). f Representative Western blots (top) and quantification (bottom) of iNOS, ARG1, and GAPDH of BMDMs exposed to DMSO or 5 µM Yoda1 and stimulated with media (Unstim.), IFNγ/LPS (0.3 ng/mL of each), or IL4/IL13 (0.1 ng/mL of each). g TNFα, IL6, and MCP1 secretion from BMDMs exposed to DMSO or 5 µM Yoda1 and incubated in the indicated conditions, as measured by ELISA. h Relative gene expression of inflammatory and healing markers of BMDMs exposed to DMSO or 5 µM Yoda1 incubated in the indicated conditions, as measured by qPCR. Gene expression is shown relative to the highest expressing condition. i–j Representative Western blots (top) and quantification (bottom) of p-NFκB/NFκB of wild-type BMDMs exposed to DMSO or Yoda1 and stimulated with media (Unstim.) or IFNγ/LPS for 2 h (d) and p-STAT6/STAT6 of Piezo1^fl/+ and Piezo1^ΔLysM BMDMs incubated with media (Unstim.) or IL4/IL13 for a period of 1 h. Error bars denote Mean ± SD for three independent experiments, * p < 0.05 as determined by two-tailed Student’s t test. Phosphorylated and total forms of transcription factors were obtained from loading equal amounts of protein in separate gels and the resulting blots were processed in parallel. Source data including exact p-values are provided as a Source Data file.