Fig. 4. Piezo1 modulates the foreign body response and macrophage activation in response to stiff material implants.

a–d Representative H&E (a) and Masson’s trichrome (c) stained tissue surrounding soft (1 kDa) and stiff (140 kDa) PEGDA material implanted in Piezo1^fl/+ and Piezo1^ΔLysM mice for a period of 14 days. Quantification of immune cell infiltrate normalized to area (b) and average collagen capsule thickness (d). N = 6 for Piezo1^fl/+ mice treated with soft and stiff implants as well as Piezo1^ΔLysM treated with soft implants, and n = 7 for Piezo1^fl/+ and Piezo1^ΔLysM mice treated with stiff implants. e, f Representative immunohistochemistry images of tissue collected 3 days (D3, e) and 14 days (D14, f) post-implantation and stained for F4/80, iNOS, ARG1, and Hoechst. g Quantification of percent cells that stained positive for F4/80. h Quantification of percent cells that stained positive for F4/80 and iNOS. i Quantification of percent cells that stained positive for F4/80 and ARG1. Material location indicated with asterisk. N = 5 and error bars denote Mean ± SD for n ≥ 5, * p < 0.05 as determined by two-tailed Student’s t test. Source data including exact p-values are provided as a Source Data file.