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. 2021 Apr 29;24:1012–1023. doi: 10.1016/j.omtn.2021.04.007

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Restored ILF3-AS1/EZH2 accelerates proliferation, colony-formation, migration, and invasion ability, as well as inhibits apoptosis of CRC cells

(A) Detection of HT29 cell growth curve by CCK-8 assay. (B) Colony-formation ability tested in HT29 cells by colony-formation assay. (C) Comparison of colony-formation number of HT29 cells in vitro. (D) Apoptosis of HT29 cells in each group. (E) Comparison of apoptosis rate in each group of HT29 cells. (F) Experimental results of scratch healing of HT29 cells in each group. (G) Comparison of scratch-healing rate of HT29 cells. (H) Invasion of HT29 cells tested by Transwell assay. (I) Comparison of invasion ability of HT29 cells in each group. dp < 0.05 versus the overexpressed (oe)-Ctr group, ep < 0.05 versus the oe-NC group, fp < 0.05 versus the oe-ILF3-AS1 group. Comparisons among multiple groups were assessed by one-way ANOVA, followed by Tukey’s multiple comparisons test for pairwise comparison.