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. 2021 Apr 29;24:1012–1023. doi: 10.1016/j.omtn.2021.04.007

Figure 6.

Figure 6

Low expression of ILF3-AS1 decreases EZH2 expression, as well as increases CDKN2A expression in LoVo cells, whereas oe-ILF3-AS1 increases EZH2 expression, as well as decreases CDKN2A expression in HT29 cells

(A) Comparison of ILF3-AS1, EZH2, and CDKN2A expression in LoVo cells tested by qRT-PCR. (B) Protein bands of EZH2 and CDKN2A in LoVo cells by western blot assay. (C) Comparison of EZH2 and CDKN2A protein expression in LoVo cells by western blot assay. (D) Comparison of ILF3-AS1, EZH2, and CDKN2A expression in HT29 cells tested by qRT-PCR. (E) Protein bands of EZH2 and CDKN2A in HT29 cells by western blot assay. (F) Comparison of EZH2 and CDKN2A protein expression in HT29 cells by western blot assay. ap < 0.05 versus the si-Ctr group, bp < 0.05 versus the si-NC group, cp < 0.05 versus the si-ILF3-AS1 group, dp < 0.05 versus the oe-Ctr group, ep < 0.05 versus the oe-NC group, fp < 0.05 versus the oe-ILF3-AS1 group. Comparisons among multiple groups were assessed by one-way ANOVA, followed by Tukey’s multiple comparisons test for pairwise comparison.