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PANC-1 2D cells, 3D spheroids and exosomes isolated from spheroids culture medium. (A) PANC-1 2D cells. Scale bar: 100 μm. (B) PANC-1 3D spheroids. A total of 5000 cells were seeded for each spheroid. Scale bar: 200 μm. (C) Typical transmission electron microscope (TEM) image of isolated exosomes secreted by PANC-1 3D spheroids. Scale bar: 100 nm. (D) The relative number of exosomes per field (n = 5) analyzed by TEM.
