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. 2021 May 8;296:100761. doi: 10.1016/j.jbc.2021.100761

Figure 2.

Figure 2

Reno-protective effect of DPPIII on diabetic mice.A, representative Coomassie Brilliant Blue staining of urine samples after 8 weeks and different amounts of bovine serum albumin (BSA). The urine samples from mice were collected for 24 h (1 day), and the proteins in the samples were separated by SDS-PAGE. An arrowhead indicates urine albumin. BSA was used as a control. B, summary graph of the amount of excreted albumin for 1 day in each group. C, confocal images of desmin and nephrin co-immunostaining in the glomeruli. Arrowheads indicate abnormal desmin deposition. D, summary graph of the percentage of desmin-positive areas per glomerulus. E, confocal images of CD31 and podocin co-immunostaining in the glomeruli. F, ultrastructures of the glomeruli observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Arrowheads indicate damaged podocyte foot processes. Scale bars: 20 μm (C and E) and 1 μm (F). In B, two-way ANOVA was applied for comparing the data between groups, and one-way ANOVA was applied for comparing the results at week 0 with those at other time points; in C, one-way ANOVA was used to compare the data of each group. ∗∗p < 0.01 versus C57BL/6 mice; †p < 0.05 versus 0 weeks; §p < 0.05 and §§p < 0.01 versus PBS-infused db/db mice.