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. 2021 Apr 15;40(11):e99692. doi: 10.15252/embj.201899692

Figure EV1. USP7 inhibitors trigger a widespread accumulation of mitotic signaling events.

Figure EV1

  •  
    Flow cytometry profiles illustrating the levels of H3S10P and MPM‐2 in cells either treated with different USP7 inhibitors (red) or with DMSO as a control (black).
  • A, B
    RPE and U2OS cells treated with 25 or 50 μM P22077 for 4 h, respectively.
  • C
    RPE cells treated with 50 μM HBX 19818 for 16 h.
  • D, E
    HCT‐116 (D) and RPE (E) cells treated with 25 μM BAY 11‐7082 for 4 h (H3S10P) and 2 h (MPM‐2).
  • F
    MPM‐2 levels in U2OS cells treated with 25 μM BAY 11‐7082 for 4 h.
  • G, H
    HCT‐116 and RPE cells treated with 60 μM FT671 for 16 h.
  • I
    RPE cells treated with 50 μM GNE‐6640 for 8 h (H3S10P) and 24 h (MPM‐2).