Figure 1. SUD interacts with Paip1.

1. Genome organization of SARS‐CoV and schematic illustration of Nsp3 domains. DPUP: domain preceding Ubl2; Mac: macrodomain; NAB: nucleic acid‐binding domain; PL^pro: papain‐like protease; Ubl1: ubiquitin‐like domain 1.
2. SUD binding to Paip1 in HEK‐293 cells as demonstrated by the split‐YFP assay.
3. Schematic presentation of SUD, Paip1, and PABP. Interactions between SUD and Paip1, as well as PAPB and Paip1 are indicated by red and black lines, respectively. PAM: PABP‐binding motif; Paip1M: the middle domain of Paip1; RRM: RNA‐recognition motif; PABC: the C‐terminal domain of PABP.
4. Paip1M alone does interact with SUD.
5. SUD binds to Paip1 and Paip1M with the same binding intensity. n. s.: not significant.
6. The Mac2 subdomain is crucial for interacting with Paip1M.
7. Paip1M and SUD interaction was confirmed in vitro by a gel filtration assay.

Data information: Scale bars represent 50 µm.