Figure 2. Inhibition of Syk‐Cn‐NFAT signaling pathway promotes chemical reprogramming.

• A
  Schematic diagram showing Syk‐Cn‐NFAT pathway axis.
• B
  Immunofluorescence of Sall4 in reprogramming intermediates treated with DMSO and FK506. n = 5. Scale bar, 100 μm.
• C, D
  Concentration (C) and stage (D) test of FK506 during reprogramming. n = 3.
• E
  RT–qPCR analysis of Sall4, Cdh1, Epcam, and Esrrb gene expression in reprogramming intermediates treated with DMSO and FK506. n = 3.
• F
  Diagram showing the procedure of Ppp3ca knockdown at the early stage of reprogramming.
• G
  RT–qPCR analysis of Ppp3ca expression in MEFs infected with shNC and shPpp3ca viruses. n = 3.
• H
  Immunofluorescence of Sall4 in WT cells and Ppp3ca knockdown cells on d12. n = 6. Scale bar, 100 μm.
• I
  RT–qPCR analysis of pluripotent genes expression in cells treated with shNC and shPpp3ca. n = 3.
• J
  Diagram showing the procedure of shNfatc1 virus infection at the early stage of reprogramming.
• K
  RT–qPCR analysis of Nfatc1 expression in MEFs infected with shNC and shNfatc1 viruses. n = 3.
• L
  Immunofluorescence of Sall4 in WT cells and Nfatc1 knockdown cells on d12. n = 3. Scale bar, 100 μm.
• M
  RT–qPCR analysis of pluripotent genes expression in cells treated with shNC and shNfatc1. n = 3.

Data information: All data are presented as mean ± SD. Statistical significance was assessed by the two‐tailed Student’s t‐test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See also Fig EV2.